Pancreatic and duodenal homeobox 1 (PDX1) phosphorylation at serine-269 is HIPK2-dependent and affects PDX1 subnuclear localization
… Rabbit polyclonal anti-PDX1 antibody was as described [18]. Anti-phospho-Ser-269-PDX1 antibody was raised in rabbits by immunization with synthetic phospho-peptide: L 262 PSGLSVpSPQPSSIAPLRPQEPR 284 (Pacific Immunology Inc, USA). …
Rong An,a1 Gabriela da Silva Xavier,a1 Francesca Semplici,a1 Saharnaz Vakhshouri,a Huai-Xiang Hao,b Jared Rutter,b Mario A. Pagano,c Flavio Meggio,c Lorenzo A. Pinna,c and Guy A. Ruttera
Biochem Biophys Res Commun. 2010 August 20; 399(2): 155–161. doi: 10.1016/j.bbrc.2010.07.035.
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Mouse Skeletal Muscle Fiber-Type-Specific Macroautophagy and Muscle Wasting Are Regulated by a Fyn/STAT3/Vps34 Signaling Pathway
… pY261-LKB1 antibody was produced by Pacific Immunology Co (Ramona, CA, USA). Leupeptin was from Fisher Bioreagents (Pittsburgh, PA, USA). All other reagents were from Sigma. pRC/CMV-Flag-S …
Eijiro Yamada1, 2, Claire C. Bastie1, 4, Hiroshi Koga3, Yichen Wang2, Ana Maria Cuervo1, 3 and Jeffrey E. Pessin
Cell Reports, Volume 1, Issue 5, 557-569, 03 May 2012
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A Conserved Threonine Residue in the Juxtamembrane Domain of the XA21 Pattern Recognition Receptor Is Critical for Kinase Autophosphorylation and XA21-mediated Immunity
… SDS-PAGE (10% gel) was used to separate the proteins. Western blotting was performed as described previously (29) using anti-LexA (Clontech), anti-XB24 (Pacific Immunology), or anti-XB15 (Pacific Immunology) antibodies. Immunoprecipitation of Rice Proteins. …
1.Xuewei Chen, 2.Mawsheng Chern, 3.Patrick E. Canlas, 4. Caiying Jiang1, 5.Deling Ruan, 6.Peijian Cao and 7.Pamela C. Ronald2
The Journal of Biological Chemistry, 285, 10454-10463. 1/29/2010
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Tyrosine Phosphorylation of the Nuclear Receptor Coactivator AIB1/SRC-3 Is Enhanced by Abl Kinase and Is Required for Its Activity in Cancer Cells
… The antibodies used for IP were 4G10 phosphorylated tyrosine antibody (Ab) agarose conjugate (Upstate Biotech, Inc.), AIB1 monoclonal antibody (MAb) (BD Transduction Laboratories), phospho-Y1357 AIB1 polyclonal Ab (Pacific Immunology Co.), FLAG M2 affinity gel (Sigma …
Annabell S. Oh,1 John T. Lahusen,1 Christopher D. Chien,1 Mark P. Fereshteh,2 Xiaolong Zhang,3 Sivanesan Dakshanamurthy,1 Jianming Xu,4 Benjamin L. Kagan,1 Anton Wellstein,1,2 and Anna T. Riegel1,2*
Molecular and Cellular Biology, November 2008, p. 6580-6593, Vol. 28, No. 21
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The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons
… To produce antibodies specific for Nek3 phosphorylated at Thr475, rabbits were immunized with peptides corresponding to Nek3 residues 466-481 [Cys-FEPRLDEEDT(-P)DFEEDN, where T(-P) represents phosphothreonine] at Pacific Immunology. …
Jufang Chang1, Robert H. Baloh2,3 and Jeffrey Milbrandt1,3,*
Journal of Cell Science 122, 2274-2282 (2009)
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