Pacific Immunology® is a USA biotech company located in San Diego, California that specializes in the production of custom antibodies for researchers around the world. Our facility, established in 1972, is fully NIH and USDA compliant and has helped tens of thousands of scientists obtain the antibodies that they need to run experiments, publish research and develop commercial diagnostic tests.
Determine The Antigen to Target
The first step in developing a custom antibody is to choose between immunizing with a peptide sequence or a full-length protein. For peptides, Pacific Immunology will analyze the protein sequence and recommend the best sequences to use. For proteins, please review the Antigen Preparation Guidelines for details on how to send the protein to us.
Once the antigen has been selected, Pacific Immunology will create a quotation based on the Package chosen, sequence(s) selected and the relevant shipping and handling costs. With this information, please complete the PDF order form and submit via email or include a copy when sending your protein to us.
Once Pacific Immunology has received or synthesized the antigen (peptides will typically take 2-3 weeks to synthesize and conjugate), immunizations can begin immediately. At this point, a copy of the Project Report will be provided, which includes the bleed/immunization protocol and the dates when shipments will occur.
Pacific Immunology typically ships serum after each bleed is collected (for international clients, serum is typically shipped in one or two shipments to reduce shipping costs). An ELISA is usually run on the first production bleed to verify the immune response. If purifications or other services have been ordered, these will be performed on the specified dates.
Exsanguination Bleeds are added to most projects and provide additional serum while automatically terminating the project. Projects can also be extended or terminated without exsanguination bleeds. Please request these services prior to the 4th production bleed to avoid additional charges.
Extend or Finish The Project
Developing a custom antibody for the first time can seem like an overwhelming task. However, by outsourcing this to the antibody experts at Pacific Immunology®, you can join thousands of other scientists who have chosen to trust our company to provide them with the antibodies that they need. This diagram gives a complete overview of what to expect from start to finish on a custom project.
With over 50 years of experience in generating custom antibodies, Pacific Immunology® offers numerous benefits for developing your custom antibody: