The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.

Heparin Binding Motifs and Biofilm Formation by Candida albicans

…Candida albicans is a leading pathogen in infections of central venous catheters, which are frequently infused with heparin. Binding of C. albicans to medically relevant concentrations of soluble and plate-bound heparin was demonstrable by confocal microscopy and ELISA assay. A sequence-based search identified 34 C. albicans surface proteins containing at least one match to linear heparin binding motifs (HBM)…
Julianne V. Green1, Kris I. Orsborn1, Minlu Zhang2, Queenie K.-G. Tan3, Kenneth D. Greis4, Aleksey Porollo5,6, David R. Andes7, Jason Lu Long6 and Margaret K. Hostetter
J Infect Dis. (2013) doi: 10.1093/infdis/jit391

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Identification and characterization of Drosophila Snurportin reveals a role for the import receptor Moleskin/Importin7 in snRNP biogenesis

…Nuclear import is an essential step in small nuclear ribonucleoprotein (snRNP) biogenesis. Snurportin1 (SPN1), the import adaptor, binds to trimethylguanosine (TMG) caps. A rabbit polyclonal anti-dSNUP antibody (dSNUP) was generated (Pacific Immunology, Ramona, CA) using GST-tagged dSNUP.
Amanda Hicks Natalizio3 and A. Gregory Matera
Mol. Biol. Cell July 24, 2013 mbc.E13-03-0118

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Functional Differentiation of Small Heat Shock Proteins in Diapause-Destined Artemia Embryos

…Encysted embryos of Artemia franciscana cease development and enter diapause, a state of metabolic suppression and enhanced stress tolerance. The development of diapause-destined Artemia embryos is characterized by the coordinated synthesis of the small heat shock proteins (sHsps) p26, ArHsp21 and ArHsp22, the latter stress inducible in adults. The amounts of sHsp mRNA and protein varied…
Allison M. King, Jantina Toxopeus, Thomas H. MacRae
DOI: 10.1111/febs.12442

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Overexpression of the Escherichia coli TolQ protein leads to a null-FtsN-like division phenotype

…Resolved proteins were transferred to a polyvinylidene fluoride membrane (Millipore Corp. Bedford, MA) and probed with a monospecific polyclonal rabbit antiserum raised against a synthetic peptide corresponding to TolQ residues 47-62 (generated by Pacific Immunology, San Diego, CA), and visualized using an anti-rabbit immunoglobulin horseradish peroxidase conjugate and enhanced chemiluminescence (ECL)…
Mary A. Teleha1,2, Adam C. Miller2, Ray A. Larsen
Microbiology Open 2 JUL 2013 DOI: 10.1002/mbo3.101

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Purification and Characterization of Shiga Toxin 2f, an Immunologically Unrelated Subtype of Shiga Toxin 2

…To develop a Stx2a polyclonal antibody, a rabbit was immunized with catalytically inactive Stx2a toxoid (E167Q mutation) and serum was collected by cardiac puncture with exsanguination (procedures performed by Pacific Immunology)…
Craig Skinner, Stephanie McMahon, Reuven Rasooly, John Mark Carter, Xiaohua He
PLoS ONE 8(3): e59760. doi:10.1371/journal.pone.0059760

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