Purification and Characterization of Shiga Toxin 2f, an Immunologically Unrelated Subtype of Shiga Toxin 2

…To develop a Stx2a polyclonal antibody, a rabbit was immunized with catalytically inactive Stx2a toxoid (E167Q mutation) and serum was collected by cardiac puncture with exsanguination (procedures performed by Pacific Immunology)…
Craig Skinner, Stephanie McMahon, Reuven Rasooly, John Mark Carter, Xiaohua He
PLoS ONE 8(3): e59760. doi:10.1371/journal.pone.0059760

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