Rice XB15, a Protein Phosphatase 2C, Negatively Regulates Cell Death and XA21-Mediated Innate Immunity
… For the anti-XB15 antibody, synthetic peptides and monospecific antibodies were made as a service by Pacific Immunology. Detailed information about their methods can be obtained at Pacific Immunology (http://www.pacificimmunology.com/). Epitope selection was directed to hydrophobic, flexible …
Chang-Jin Park1, Ying Peng1, Xuewei Chen1, Christopher Dardick2, DeLing Ruan1, Rebecca Bart1, Patrick E. Canlas1, Pamela C. Ronald1*
BioCreative, September 2008 | Volume 6 | Issue 9 | e231
Cdc14-Dependent Dephosphorylation of a Kinetochore Protein Prior to Anaphase in Saccharomyces cerevisiae
… Dsn1–Ser264, a Cdk1 target site, is hypophosphorylated prior to anaphase. (A) A polyclonal phospho-specific antibody was custom-made by Pacific Immunology against phospho-Ser264 Dsn1 using peptide GGSTII(pS)PHKDIPEED. …
Bungo Akiyoshi*, and Sue Biggins*,1
Genetics, Vol. 186, 1487-1491, December 2010, doi:10.1534/genetics.110.123653
Autoregulation of Fox protein expression to produce dominant negative splicing factors
… MATERIALS AND METHODS. Antibodies. Rabbit polyclonal antibody (Pacific Immunology) was raised to a Fox-2 fragment containing amino acid residues 120–205 and affinity purified on antigen. This antibody specifically recognizes …
Andrey Damianov and Douglas L. Black
RNA 2010 16: 405-416
A Ral GAP complex links PI 3-kinase/Akt signaling to RalA activation in insulin action
… The Myr-Akt-HA construct was kindly provided by A. Vojteck (University of Michigan). All other constructs were described previously (Chen et al., 2007). Antibodies. Rabbit polyclonal antibodies were generated by Pacific Immunology (Ramona, CA). …
Xiao-Wei Chena,b,*, Dara Letoa,c,*, Tingting Xionga,b, Genggeng Yua, Alan Chenga, Stuart Deckera, and Alan R. Saltiela,b,c,
Molecular Biology of the Cell, December 9, 2010 Vol. 22, Issue 1, 141-152
Phosphorylation of connexin43 on serine 306 regulates electrical coupling
… affinity purified rabbit antibody directed against phosphorylated serine 306 in Cx43 (pS306 Ab, Pacific Immunology, Ramona, CA). The antibody was raised against the following sequence: CRNYNKQA-S(PO3)-EQNWAN, which spans …
Kristina Procida MD1, Lone Jørgensen MS1, Nicole Schmitt PhD1, Mario Delmar MD, PhD2, Steven M. Taffet PhD3, Niels-Henrik Holstein-Rathlou MD, PhD1, Morten Schak Nielsen PhD1, and Thomas Hartig Braunstein PhD1
DOI: 10.1016/j.hrthm.2009.07.043