Hsp90 regulates NADPH oxidase activity and is necessary for superoxide but not hydrogen peroxide production

… subjected to western blot analysis. Nox5 phosphorylation state-specific antibodies to Ser 490 , Thr 494 , and Ser 498 were generated by Pacific Immunology as previously described (22). BRET assay Forty-eight hours after transfection …
F Chen, D Pandey, A Chadli… –
… & Redox Signaling, 2011 – online.liebertpub.com

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The rapid activation of N-Ras by [alpha]-thrombin in fibroblasts is mediated by the specific G-protein G [alpha] i2-G [beta] 1-G [gamma] 5 and occurs in lipid rafts

… 371751 and 371754), α-Gα 13 (Calbiochem, 371729), α-Gβ 1 (Calbiochem, 371813), α-Gβ 3 (Calbiochem, 371819), α-Gβ 4 (Santa Cruz, sc-382), α-Gβ 5 (Calbiochem, 371819), α-Gγ 5 (Santa Cruz, sc-376), α-Gγ 7 (Santa Cruz, sc-377), α-Gγ 11 (Pacific Immunology), α-GRF2 …
Nathan H. Lentsb, , , Virginia Irintchevaa, Reema Goela, Leroy W. Wheelera, Joseph J. Baldassare
Cellular signalling, 2009 – Elsevier

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MAP Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation and Cell Adhesion

… dishes for 3 or 24 hrs before harvest (1). 103 Antibodies and recombinant proteins. Generation of peptides and affinity purified 104 polycolonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology 105 (Ramona, CA). …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol
http://mcb.asm.org/content/early/2012/05/15/MCB.06327-11.abstract

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Mitogen-Activated Protein Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation, and Cell …

… before harvest (1). Antibodies and recombinant proteins.Generation of peptides and affinity-purified polyclonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology (Ramona, CA). Peptides used …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol

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Characterization of the Cellular and Antitumor Effects of MPI-0479605, a Small-Molecule Inhibitor of the Mitotic Kinase Mps1

… Thermo Scientific). Antibodies. The Mps1-pT676 antibody was raised in rabbits using the peptide IANQMQPDTpTSVVKDSQV coupled to keyhole limpet hemocyanin as antigen (Pacific Immunology). Phosphospecific polyclonal …
KD Tardif, A Rogers, J Cassiano, BL Roth… –
Molecular cancer …, 2011 – AACR

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