Protein Kinase G Phosphorylates Soluble Guanylyl Cyclase on Serine 64 and Inhibits Its Activity
… The membranes were then blocked and incubated with the primary and secondary antibodies. The phospho-specific sGC Ab was developed by Pacific Immunology, Inc (Ramona, CA, USA) against the following sequence SHPQRKTS*RNRVYLH. …
Zongmin Zhou ; Nazish Sayed ; Anastasia Pyriochou ; Charis Roussos ; David Fulton ; Annie Beuve ; and Andreas Papapetropoulos
Arteriosclerosis, Thrombosis, and Vascular Biology: July 17, 2008, doi: 10.1161/ATVBAHA.108.165043
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Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus
… 3 (Upstate). Antibodies recognizing phosphorylated PLK3 on serine 284 were generated by Pacific Immunology (Ramona, CA) by injecting rabbits with the synthetic peptide CIKQVHYTLPASLS(P) LPAR. Non-phosphorylation …
Feng Yang1, David G. Camp, II1, Marina A. Gritsenko1, Quanzhou Luo1, Ryan T. Kelly1, Therese R. W. Clauss1, William R. Brinkley2, Richard D. Smith1 and David L. Stenoien3,*
Journal of Cell Science 120, 4060-4070 (2007)
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Identification of ChChd3 as a Novel Substrate of the cAMP-dependent Protein Kinase (PKA) Using an Analog-sensitive Catalytic Subunit
… QAKKESEHQRRLKQARDLERER (Invitrogen). Affinity-purified polyclonal antibodies to phospho-ChChd3 Thr 10 were generated in rabbits against an N-terminal peptide RRVT(PO 3 )FEADENENC (Pacific Immunology Corp, Ramona, CA). Mitochondria …
Sharmin Schauble1, Charles C. King, Manjula Darshi, Antonius Koller, Kavita Shah, and Susan S. Taylo
J. Biol. Chem., Vol. 282, Issue 20, 14952-14959, May 18, 2007
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M. tuberculosis Ser/Thr Protein Kinase D Phosphorylates an Anti-Anti–Sigma Factor Homolog
… After electrophoresis in 10%–20% Tris-glycine gels, proteins were transferred to PVDF membrane, and detected with anti-phosphoThreonine (Invitrogen), anti-DDDDK (AbCam), anti-KatG, or anti-PknD antibodies (Pacific Immunology, http://www.pacificimmunology.com). …
Andrew E. Greenstein1, Jason A. MacGurn2, Christina E. Baer3, Arnold M. Falick4, Jeffery S. Cox2, Tom Alber1
PLoS Pathog 3(4): e49. doi:10.1371/journal.ppat.0030049
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Epidermal Growth Factor-Dependent Phosphorylation of the GGA3 Adaptor Protein Regulates Its Recruitment to Membranes
… Princeton, NJ), respectively. A rabbit polyclonal antibody to CI-MPR was raised against the C-terminal peptide (NH 2 -CSFHDDSDEDLLHI-COOH) of human CI-MPR (Pacific Immunology Corp., Ramona,CA). A rabbit polyclonal …
Satoshi Kametaka, Rafael Mattera, and Juan S. Bonifacino*
Molecular and Cellular Biology, September 2005, p. 7988-8000, Vol. 25, No. 18
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