Our Custom Polyclonal Antibody Production package includes everything needed to obtain custom antibodies against an antigen that the client provides.
|Package #1: Rabbits||13-week Antibody Production Protocol:
|Package #1: Chickens||13-week Antibody Production Protocol:
|Package #1: Goats||13-week Antibody Production Protocol:
|Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals)||$195.00|
|Provides 1 immunization and 2 additional test bleeds per animal. All collected egg yolks provided. (Covers both animals)||$195.00|
|Provides 1 immunization and 2 additional production bleeds. Provides approximately 500 ml of serum.||$595.00|
|Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals)||$100.00|
|Sodium Azide||Preservative for antiserum||No Charge|
Polyclonal antibodies can be generated against both soluble and non-soluble recombinant proteins, fusion proteins, gel strips, existing peptide, etc. Live or toxic substances cannot be accepted.
We recommend sending 2-3 mg of the antigen for a rabbit or chicken project and 5-7 mg for a goat project. Extensions or immunochemistry services may require additional antigen.
Pacific Immunology® can work with most protein concentrations as long as this information is provided. 1 mg/ml is a good target range, but higher concentrations are welcome. We consider 0.25 to .5 mg/ml a minimum concentration in most cases to ensure maximum protein volumes in each immunization.
Considerations for Gel Bands:
Immunizing with gel bands works well for generating polyclonal antibodies against a purified protein. If providing a gel strip, please stain with coomassie blue, de-stain, rinse and cut out the strip containing the target protein. Please avoid freezing or homogenizing the gel strip so that we can aliquot appropriate quantities of the protein for each immunization. With gel bands in particular, it is important to maintain a higher concentration of the protein as outlined above.
Shipping Antigens to Pacific Immunology®:
Please ship the antigen in a Styrofoam box with ice packs (dry ice isn’t necessary) via FedEx, UPS or DHL Overnight to the address listed on our Order Form. To avoid additional shipping charges by these companies, please insert the Styrofoam box into a cardboard box or shipping pak prior to shipping. Please also wrap paraffin around the vial containing the antigen and place the vial into a ziploc bag to contain any leakage. Please also include a printed copy of the Order Form with the shipment.