Custom Monospecific Antibody Production is our most popular package and can serve as a cost effective alternative to monoclonal antibody production for many applications. By immunizing with a peptide sequence and then affinity purifying the serum against that peptide sequence, it is possible to isolate highly specific antibodies that approach the specificity of monoclonal antibodies but provide the superior affinity of polyclonal antibodies.
|Package #3: Rabbits||
|Peptide||Cost per each additional peptide residue over the 15 residues included with the package||$20.00|
|Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals)||$180.00|
|Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals)||$60.00|
|200 ml Affinity Purification||Upgrade of 25 ml Affinity Purification to 200 ml Affinity Purification||$400.00|
|Sodium Azide||Preservative for antiserum||No Charge|
Pacific Immunology® provides antigen design assistance at no additional charge. We use our innovative and proprietary combination of algorithms to analyze your protein sequence and predict which regions are immunogenic, unique and exposed.
Conjugation of Peptide to Carrier Protein:
Because peptides are too small to generate an immune response by themselves, conjugation to a carrier protein will be required for immunizations. Pacific Immunology® recommends coupling peptides to KLH (Keyhole Limpet Hemocyanin).
Pacific Immunology® recommends coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. For this reason, we recommend avoiding sequences that contain internal cysteines whenever possible.
Quantity of Peptide:
Pacific Immunology® typically synthesizes 40-50 mg of immunograde peptide, with approximately 10 mg being required for conjugation to the carrier protein, ELISA and affinity purification. All unused peptide will be delivered to the customer.
The affinity column is included so that additional purifications can be run as needed. Longevity of the columns varies, but best yields are typically obtained within 6-12 months of the project’s completion.
Pacific Immunology® offers one of the highest titer guarantees in the industry, promising at least a 1:50,000 antibody titer against the peptide sequence. For additional details, please read our Guarantee page in our Resources section.
Purified Antibody Yields:
On average, 2-3 mg of peptide specific antibody will be isolated from affinity purification of 25 ml of antiserum.
Affinity Purification Strategy:
Unless one animal has particularly high antibody titers against the peptide, we recommend affinity-purifying a pool of 25 ml serum taken in a 1:1 ratio from both animals to ensure maximum epitope variability. Comprehensive ELISA testing helps in determining this strategy and in confirming the success of the purification.
Comparison to Monoclonal Antibodies:
Monospecific antibodies offer significant price and time advantages in comparison to monoclonal antibodies. In particular, the affinity purified pool of antibodies against the peptide sequence is highly specific and approaches a monoclonal antibody. At the same time, monospecific antibodies deliver the superior affinity of polyclonal antibodies, which allows for more robust detection of the protein in various assays and conditions. With large scale affinity purifications, large quantities of purified antibody can be obtained.