Custom Monospecific Antibody Production is our most popular package and can serve as a cost effective alternative to monoclonal antibody production for many applications. By immunizing with a peptide sequence and then affinity purifying the serum against that peptide sequence, it is possible to isolate highly specific antibodies that approach the specificity of monoclonal antibodies but provide the superior affinity of polyclonal antibodies.

Service Details Price
Package #3: Rabbits
    • Protein Sequence Analysis
    • Synthesis of up to a 15aa peptide
    • Conjugation of 3-5 mg of the peptide to KLH
    • 13-week Antibody Production Protocol:
      • 2 Rabbits
      • Pre-Immune Serum from each animal
      • 4 Immunizations per animal
      • 4 Production Bleeds of 20-25 ml of serum per animal
      • Provides approximately 160-200 ml of anti-sera
    • ELISA on 1st Production Bleed (covers both animals)
    • Affinity Purification of 25 ml of Serum from 3rd Production Bleed
    • Reusable Affinity Column
    • Handling / Shipment Preparation
    • $200.00 Package Discount
Service Details Price
Peptide Cost per each additional peptide residue over the 15 residues included with the package $20.00
4-Week Extension
Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals) $245.00
Exsanguination Bleeds
Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals) $100.00
200 ml Affinity Purification Upgrade of 25 ml Affinity Purification to 200 ml Affinity Purification $400.00
Sodium Azide Preservative for antiserum No Charge

Antigen Design:
Pacific Immunology® includes antigen design assistance with this package service.  We use our innovative and proprietary suite of algorithms to analyze your protein sequence and predict which regions are immunogenic, unique and exposed.

Conjugation of Peptide to Carrier Protein:
Because peptides are too small to generate an immune response by themselves, conjugation to a carrier protein will be required for immunizations. Pacific Immunology® recommends coupling peptides to KLH (Keyhole Limpet Hemocyanin).

Terminal Cysteine:
Pacific Immunology® recommends coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. For this reason, we recommend avoiding sequences that contain internal cysteines whenever possible.

Quantity of Peptide:
Pacific Immunology® typically synthesizes 40-50 mg of immunograde peptide, with approximately 10 mg being required for conjugation to the carrier protein, ELISA and affinity purification. All unused peptide will be delivered to the customer.

Affinity Column:
The affinity column is included so that additional purifications can be run as needed. Longevity of the columns varies, but best yields are typically obtained within 6-12 months of the project’s completion.

Titer Guarantees:
Pacific Immunology® offers one of the highest titer guarantees in the industry, promising titers of at least 1:100,000 against the standalone peptide. For additional details, please read our Guarantee.

Purified Antibody Yields:
For most projects, 2-3 mg of peptide-specific antibody will be isolated from an affinity purification of 25 ml of antiserum.

Affinity Purification Strategy:
Unless one animal has particularly high antibody titers against the peptide, we recommend affinity-purifying a pool of 25 ml serum mixed from both animals to ensure maximum epitope variability. Comprehensive ELISA testing helps in determining this strategy and in confirming the success of the purification.

Comparison to Monoclonal Antibodies:
Monospecific antibodies offer significant price and time benefits in comparison to monoclonal antibodies. In particular, the affinity purified pool of antibodies against the peptide sequence is highly specific and approaches the specificity of a monoclonal antibody. At the same time, monospecific antibodies deliver the typically higher affinity of polyclonal antibodies, which allows for more robust detection of the protein in various assays and conditions. With large scale affinity purifications, large quantities of purified antibody can be obtained.