Overview:
Our Custom Peptide Antibody Production package includes everything needed to obtain custom antibodies against a synthesized peptide. Please note that ELISA and Affinity Purification of the serum against the peptide is not included with this package. For the best results, we recommend our Monospecific Antibody Production Package, which includes these services with a package pricing discount. Affinity purification of the serum against the peptide will eliminate non-specific cross reactivity in the serum and maximize specificity of the anti-peptide antibodies in your assays.

Service Details Price
Package #2: Rabbits

  • Protein Sequence Analysis
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • Handling / Shipment Preparation
  • 13-week Antibody Production Protocol:
    • 2 Rabbits
    • Pre-Immune Serum from each animal
    • 4 Immunizations per animal
    • 4 Production Bleeds of 20-25 ml of serum per animal
    • Provides approximately 160-200 ml of anti-sera

$1095.00
Package #2: Chickens

  • Protein Sequence Analysis
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • Handling / Shipment Preparation
  • 13-week Antibody Production Protocol:
    • 2 Chickens
    • Pre-Immune Serum from each animal
    • 4 Immunizations per animal
    • 4 Test Bleeds of 1 ml of serum per animal
    • Yolks from all eggs collected
    • Provides approximately 160-200 ml of egg yolk

$1095.00
Package #2: Goats

  • Protein Sequence Analysis
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • Handling / Shipment Preparation
  • 13-week Antibody Production Protocol:
    • 1 Goat
    • Pre-Immune Serum
    • 4 Immunizations
    • 4 Production Bleeds of approximately 250 ml
    • Provides approximately 1000 ml of anti-sera

$1495.00
Service Details Price
Peptide Cost per each additional peptide residue over the 15 residues included with the package $20.00
4-Week Extension
(Rabbits)
Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals) $180.00
4-Week Extension
(Chickens)
Provides 1 immunization and 2 additional test bleeds per animal. All collected egg yolks provided. (Covers both animals) $180.00
4-Week Extension
(Goats)
Provides 1 immunization and 2 additional production bleeds. Provides approximately 500 ml of serum. $375.00
Exsanguination Bleeds
(Rabbits)
Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals) $60.00
Sodium Azide Preservative for antiserum No Charge

Antigen Design:
Pacific Immunology® provides antigen design assistance at no additional charge. We use our innovative and proprietary combination of algorithms to analyze your protein sequence and predict which regions are immunogenic, unique and exposed.

Conjugation of Peptide to Carrier Protein:
Because peptides are too small to generate an immune response by themselves, conjugation to a carrier protein will be required for immunizations. Pacific Immunology® recommends coupling peptides to KLH (Keyhole Limpet Hemocyanin).

Terminal Cysteine:
Pacific Immunology® recommends coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. For this reason, we recommend avoiding sequences that contain internal cysteines whenever possible.

Quantity of Peptide:
Pacific Immunology® typically synthesizes 40-50 mg of immunograde peptide, with 3-5 mg being required for conjugation to the carrier protein. All unused peptide will be delivered to the customer.

Titer Guarantees:
Pacific Immunology® offers one of the highest titer guarantees in the industry, promising at least a 1:50,000 antibody titer against the peptide sequence. For additional details, please read our Guarantee page in our Resources section.