Custom Peptide Antibody Production

Package #2: Custom Peptide Antibody Production

Overview:

The Custom Peptide Antibody Production package is designed for generating antibodies against a synthesized peptide.

Antigen Design:

Pacific Immunology provides antigen design assistance at no additional charge. Please send your sequence(s) to our Sales team with any details on regions to target/avoid, and we will be happy to send our recommendations. Once the final sequence(s) has been chosen, we can then put together a formal quotation for the project.

Conjugation of Peptide to Carrier Protein:

Because peptides are too small to generate an immune response by themselves, conjugation to a carrier protein will be required for immunizations. Pacific Immunology recommends coupling peptides to KLH (Keyhole Limpet Hemocyanin).

Terminal Cysteine:

Pacific Immunology recommends coupling the peptide to the carrier protein via a cysteine that has been added to the N or C-terminal of the peptide. For this reason, we recommend avoiding sequences that contain internal cysteines whenever possible.

Quantity of Peptide:

Pacific Immunology typically synthesizes 40-50 mg of immunograde peptide, with 3-5 mg being required for conjugation to the carrier protein. All unused peptide will be delivered to the customer.

Titer Guarantees:

Pacific Immunology offers one of the highest titer guarantees in the industry, promising at least a 1:20,000 antibody titer against the peptide sequence.

Package Details:

Service Details Price
Package #2: Rabbits
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • 13-week Antibody Production Protocol:
    • 2 Rabbits
    • Pre-Immune Serum from each animal
    • 4 Immunizations per animal
    • 4 Production Bleeds of 20-25 ml of serum per animal
    • Provides approximately 160-200 ml of anti-sera
 $995.00
Package #2: Chickens
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • 13-week Antibody Production Protocol:
    • 2 Chickens
    • Pre-Immune Serum from each animal
    • 4 Immunizations per animal
    • 4 Test Bleeds of 1 ml of serum per animal
    • Yolks from all eggs collected
    • Provides approximately 160-200 ml of egg yolk
 $995.00
Package #2: Goats
  • Synthesis of up to a 15aa peptide
  • Conjugation of 3-5 mg of the peptide to KLH
  • 13-week Antibody Production Protocol:
    • 1 Goat
    • Pre-Immune Serum
    • 4 Immunizations
    • 4 Production Bleeds of approximately 250 ml
    • Provides approximately 1000 ml of anti-sera
 $1295.00

Additional Services:

Service Details Price
Peptide Cost per each additional peptide residue over the 15 residues included with the package $20.00
4-Week Extension (Rabbits) Provides 1 immunization and 2 additional production bleeds per animal. Provides approximately 80-100 ml of serum. (Covers both animals) $150.00
4-Week Extension (Chickens) Provides 1 immunization and 2 additional test bleeds per animal. All collected egg yolks provided. (Covers both animals) $150.00
4-Week Extension (Goats) Provides 1 immunization and 2 additional production bleeds. Provides approximately 500 ml of serum. $275.00
Exsanguination Bleeds (Rabbits) Terminal Bleeds that provide approximately 50+ ml of serum per animal. (Covers both animals) $50.00
Sodium Azide Preservative for antiserum No Charge