Antibody Production Protocol
Overview:
Following is an example of our 13-week Antibody Production Protocol in Rabbits:
| Antibody Production Schedule: | Serum volume | ||
|---|---|---|---|
| Week 0 | Bleed | Pre-immune serum collection. |
~ 5 ml serum / rabbit |
| Week 0 | Immunize | AdjuLite® Complete Freund’s Adjuvant (CFA). | |
| Week 3 | Immunize | AdjuLite® Incomplete Freund’s Adjuvant (IFA). | |
| Week 6 | Immunize | AdjuLite® Incomplete Freund’s Adjuvant (IFA). | |
| Week 7 | Bleed | #1 - Production bleed. |
~ 25 ml serum / rabbit |
| Week 9 | Bleed | #2 - Production bleed. |
~ 25 ml serum / rabbit |
| Week 10 | Immunize | AdjuLite® Incomplete Freund’s Adjuvant (IFA). | |
| Week 11 | Bleed | #3 - Production bleed. |
~ 25 ml serum / rabbit |
| Week 13 | Bleed | #4 - Production bleed. |
~ 25 ml serum / rabbit |