The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.

Distinct immunoregulatory properties of macrophage migration inhibitory factors encoded by Eimeria parasites and their chicken host

… Immobilon-P, Millipore, Billerica, MA), and the membranes were treated with SuperBlock buffer (Fisher, Pittsburgh, PA) at 4°C for 16–24 h and incubated with rabbit antisera commercially prepared against recombinant E.MIF or C.MIF proteins (Pacific Immunology, Ramona, CA …
SI Jang, HS Lillehoj, SH Lee, DK Kim, M Pagés… –
Vaccine, 2011 – Elsevier

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Mitogen-Activated Protein Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation, and Cell …

… before harvest (1). Antibodies and recombinant proteins.Generation of peptides and affinity-purified polyclonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology (Ramona, CA). Peptides used …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol

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The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization

… 14). Western blotting was performed as described previously (8), using purified Pdi1p or PDIa′ as standards against customer-made yeast PDIa′ antiserum (Pacific Immunology Corp.) and anti-PGK (Invitrogen) as a control. …
JH Kim, Y Zhao, X Pan, X He, HF Gilbert –
Journal of Biological Chemistry, 2009 – ASBMB

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Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge

… After labeling for BDA, sections were labeled using an antibody directed against RFRP (1:10,000; Pacific Immunology 123/124) as previously described and validated (21) with CY-3 donkey antirabbit (1:200; Jackson ImmunoResearch, West Grove, PA) as the secondary …
Erin M. Gibson, Stephanie A. Humber, Sachi Jain, Wilbur P. Williams III, Sheng Zhao, George E. Bentley, Kazuyoshi Tsutsui and Lance J. Kriegsfeld
Endocrinology, 2008 – Endocrine Soc

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Characterization of the Cellular and Antitumor Effects of MPI-0479605, a Small-Molecule Inhibitor of the Mitotic Kinase Mps1

… Thermo Scientific). Antibodies. The Mps1-pT676 antibody was raised in rabbits using the peptide IANQMQPDTpTSVVKDSQV coupled to keyhole limpet hemocyanin as antigen (Pacific Immunology). Phosphospecific polyclonal …
KD Tardif, A Rogers, J Cassiano, BL Roth… –
Molecular cancer …, 2011 – AACR

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