The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.

The tubulin-like RepX protein encoded by the pXO1 plasmid forms polymers in vivo in Bacillus anthracis

… sulfate-polyacrylamide gel electrophoresis. The purified protein was used to raise anti-RepX polyclonal antibodies in rabbits, and the antibodies were affinity purified (Pacific Immunology, Ramona, CA). For immunoblot analysis …
P Akhtar, SP Anand, SC Watkins… –
Journal of bacteriology, 2009 – Am Soc Microbiol

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MAP Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation and Cell Adhesion

… dishes for 3 or 24 hrs before harvest (1). 103 Antibodies and recombinant proteins. Generation of peptides and affinity purified 104 polycolonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology 105 (Ramona, CA). …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol

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Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

… 1 mm glass beads, Biospec Products, 11079110, 70 μm cell strainer, BD Falcon, 352350, 96-well plate, Costar, 3799, Adjulite Incomplete Freund’s Adjuvant, Pacific Immunology, n/a, DMSO, Sigma, D-5879, FBS, GIBCO, 16000-077, FC 500 Flow Cytometer, Beckman Coulter, n/a …
D Marina, H Jerome, S Gary –
Journal of Visualized Experiments, 2010 –

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PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma

… for Western blotting: glyceraldehyde 3-phosphate dehydrogenase (GAPDH, P04406, Millipore, Billerica, MA), β-actin (sc-1615, Santa Cruz, Santa Cruz, CA), PEA15 (against PEA15 C-terminal peptide sequence NH 2 -PSEEEIIKLAPPPKKA, Pacific Immunology, Ramona, CA …
JE Gawecka, D Geerts, J Koster… – …
Journal of Cancer, 2011 – Wiley Online Library

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The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides

… 2.5. Immunoblotting. Peptide antibodies were designed to specifically recognize GSTE2 and GSTE7. Peptide synthesis, generation of polyclonal antibodies in rabbits and affinity purification was performed by Pacific Immunology Corporation, USA. …
N Lumjuan, S Rajatileka, D Changsom… –
Insect Biochemistry and …, 2011 – Elsevier

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