Introduction

The following citations highlight custom antibodies that have been developed by Pacific Immunology® and successfully used by researchers to publish their research in peer-reviewed journals.


The stability and activity of respiratory Complex II is cardiolipin-dependent

… Immunoblots were conducted with primary antibodies against Sdh1p (αSdh1p, a generous gift of Dr. Bernard Lemire) and Sdh3p (αSdh3p, custom made, Pacific Immunology) with Amersham ECL Plex Cy5-conjugated secondary antibody. …
CT Schwall, VL Greenwood, NN Alder –
Biochimica et Biophysica Acta (BBA …, 2012 – Elsevier

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Amblyomma americanum (L.)(Acari: Ixodidae) tick salivary gland serine protease inhibitor (serpin) 6 is secreted into tick saliva during tick feeding

… Production of rabbit polyclonal antibodies to rAamS6. The production of rabbit polyclonal antibodies to the rAamS6 protein fused with a 6-His tag at the amino-terminal region was subcontracted to a fee-for-service company (Pacific Immunology, Ramona, CA, USA). …
KC Chalaire, TK Kim, H Garcia-Rodriguez… –
Journal of …, 2011 – jeb.biologists.org

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Sinorhizobium meliloti ExoR is the target of periplasmic proteolysis

… Purification of the His-tagged ExoR protein was confirmed using Coomassie blue-stained SDS-polyacrylamide gels and sent to the Pacific Immunology Company (Ramona, CA) to raise ExoR-specific polyclonal antibodies in rabbits. …
HY Lu, L Luo, MH Yang, HP Cheng –
Journal of Bacteriology, 2012 – Am Soc Microbiol

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Inhibitor of Nrf2 (INrf2 or Keap1) Protein Degrades Bcl-xL via Phosphoglycerate Mutase 5 and Controls Cellular Apoptosis

… catalog no. ALX-201073). We generated and purified bacterial PGAM5L-His-tagged protein. The polyclonal antibodies against the PGAM5L form were generated in rabbits (Pacific Immunology) and purified. The membranes …
SK Niture, AK Jaiswal –
Journal of Biological Chemistry, 2011 – ASBMB

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Expression of the tumour antigen T21 is up‐regulated in prostate cancer and is associated with tumour stage

… 3 A mono-specific T21 334 – 351 antibody was prepared in rabbits on request by Pacific Immunology, USA against the following T21-derived peptide epitope; CVELERQLRKENEKQKNEL. The irrelevant peptide sequence is as follows: CLHGDREQRDREKALEN. …
AK Miles, A Rogers, T McCulloch, Z Hodi… –
BJU …, 2011 – Wiley Online Library

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