Quantification of Thyroglobulin, a Low-Abundance Serum Protein, by Immunoaffinity Peptide Enrichment and Tandem Mass Spectrometry

… The peptide-KLH conjugates were combined and used to immunize 2 New Zealand White rabbits (standard protocol; Pacific Immunology). peptide-conjugate beads, affinity purification of antibody, and antipeptide paramagnetic beads. …
Andrew N. Hoofnagle1,a, Jessica O. Becker1, Mark H. Wener1 and Jay W. Heinecke2
Clinical Chemistry 54: 1796-1804, 2008. September 18, 2008; 10.1373/clinchem.2008.109652 (Clinical Chemistry. 2008;54:1796-1804.)

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