Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis

…The rabbit antibody to ABCG4 was custom made by Pacific Immunology (CA, USA) against a synthetic ABCG4 peptide (KKVENHITEAQRFSHLPKR). Monospecific anti-peptide antibodies werepurified using a peptide-affinity column. The specificity of the antibody forABCG4 protein was assessed by immunofluorescence microscopy, which showed specific immunofluorescence signals in HEK293 cells expressing ABCG4 but notHEK293 cells transfected with mock vectors…
Andrew J Murphy, Nora Bijl, Laurent Yvan-Charvet, Carrie B Welch, Neha Bhagwat, Adili Reheman, Yiming Wang, James A Shaw, Ross L Levine, Heyu Ni, Alan R Tall & Nan Wang
Nature Medicine 19, 586–594 (2013) doi:10.1038/nm.3150

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