MAP Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation and Cell Adhesion
… dishes for 3 or 24 hrs before harvest (1). 103 Antibodies and recombinant proteins. Generation of peptides and affinity purified 104 polycolonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology 105 (Ramona, CA). …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol
http://mcb.asm.org/content/early/2012/05/15/MCB.06327-11.abstract
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma
… for Western blotting: glyceraldehyde 3-phosphate dehydrogenase (GAPDH, P04406, Millipore, Billerica, MA), β-actin (sc-1615, Santa Cruz, Santa Cruz, CA), PEA15 (against PEA15 C-terminal peptide sequence NH 2 -PSEEEIIKLAPPPKKA, Pacific Immunology, Ramona, CA …
JE Gawecka, D Geerts, J Koster… – …
Journal of Cancer, 2011 – Wiley Online Library
The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides
… 2.5. Immunoblotting. Peptide antibodies were designed to specifically recognize GSTE2 and GSTE7. Peptide synthesis, generation of polyclonal antibodies in rabbits and affinity purification was performed by Pacific Immunology Corporation, USA. …
N Lumjuan, S Rajatileka, D Changsom… –
Insect Biochemistry and …, 2011 – Elsevier
Mitogen-Activated Protein Kinase Phosphorylation of Splicing Factor 45 (SPF45) Regulates SPF45 Alternative Splicing Site Utilization, Proliferation, and Cell …
… before harvest (1). Antibodies and recombinant proteins.Generation of peptides and affinity-purified polyclonal antibodies to SPF45 and phospho-SPF45 were performed at Pacific Immunology (Ramona, CA). Peptides used …
AM Al-Ayoubi, H Zheng, Y Liu, T Bai… –
Molecular and Cellular …, 2012 – Am Soc Microbiol
Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge
… After labeling for BDA, sections were labeled using an antibody directed against RFRP (1:10,000; Pacific Immunology 123/124) as previously described and validated (21) with CY-3 donkey antirabbit (1:200; Jackson ImmunoResearch, West Grove, PA) as the secondary …
Erin M. Gibson, Stephanie A. Humber, Sachi Jain, Wilbur P. Williams III, Sheng Zhao, George E. Bentley, Kazuyoshi Tsutsui and Lance J. Kriegsfeld
Endocrinology, 2008 – Endocrine Soc